RESUMO
The diversity of thermophilic bacteria was investigated in four hot springs, three salt marshes and 12 desert sites in Morocco. Two hundred and forty (240) thermophilic bacteria were recovered, identified and characterized. All isolates were Gram positive, rod-shaped, spore forming and halotolerant. Based on BOXA1R-PCR and 16S rRNA gene sequencing, the recovered isolates were dominated by the genus Bacillus (97.5%) represented by B. licheniformis (119), B. aerius (44), B. sonorensis (33), B. subtilis (subsp. spizizenii (2) and subsp. inaquosurum (6)), B. amyloliquefaciens (subsp. amyloliquefaciens (4) and subsp. plantarum (4)), B. tequilensis (3), B. pumilus (3) and Bacillus sp. (19). Only six isolates (2.5%) belonged to the genus Aeribacillus represented by A. pallidus (4) and Aeribacillus sp. (2). In this study, B. aerius and B. tequilensis are described for the first time as thermophilic bacteria. Moreover, 71.25%, 50.41% and 5.41% of total strains exhibited high amylolytic, proteolytic or cellulolytic activity respectively.
Assuntos
Bacillaceae/classificação , Bacillaceae/isolamento & purificação , Bacilos Gram-Positivos/classificação , Bacilos Gram-Positivos/isolamento & purificação , Fontes Termais/microbiologia , Microbiologia do Solo , Microbiologia da Água , Bacillaceae/genética , Bacillaceae/efeitos da radiação , Biodiversidade , Análise por Conglomerados , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Bacilos Gram-Positivos/genética , Bacilos Gram-Positivos/efeitos da radiação , Dados de Sequência Molecular , Marrocos , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Esporos Bacterianos/citologiaRESUMO
The diversity of thermophilic bacteria was investigated in four hot springs, three salt marshes and 12 desert sites in Morocco. Two hundred and forty (240) thermophilic bacteria were recovered, identified and characterized. All isolates were Gram positive, rod-shaped, spore forming and halotolerant. Based on BOXA1R-PCR and 16S rRNA gene sequencing, the recovered isolates were dominated by the genus Bacillus (97.5%) represented by B. licheniformis (119), B. aerius (44), B. sonorensis (33), B. subtilis (subsp. spizizenii (2) and subsp. inaquosurum (6)), B. amyloliquefaciens (subsp. amyloliquefaciens (4) and subsp. plantarum (4)), B. tequilensis (3), B. pumilus (3) and Bacillus sp. (19). Only six isolates (2.5%) belonged to the genus Aeribacillus represented by A. pallidus (4) and Aeribacillus sp. (2). In this study, B. aerius and B. tequilensis are described for the first time as thermophilic bacteria. Moreover, 71.25%, 50.41% and 5.41% of total strains exhibited high amylolytic, proteolytic or cellulolytic activity respectively.
Assuntos
Bacillaceae/classificação , Bacillaceae/isolamento & purificação , Bacilos Gram-Positivos/classificação , Bacilos Gram-Positivos/isolamento & purificação , Fontes Termais/microbiologia , Microbiologia do Solo , Microbiologia da Água , Biodiversidade , Bacillaceae/genética , Bacillaceae/efeitos da radiação , Análise por Conglomerados , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Bacilos Gram-Positivos/genética , Bacilos Gram-Positivos/efeitos da radiação , Dados de Sequência Molecular , Marrocos , Filogenia , /genética , Análise de Sequência de DNA , Esporos Bacterianos/citologiaRESUMO
In the course of investigations realized by us earlier it was found that there was no difference between radioresistance of microbes taken from various water sources. As a matter of fact quality of the microflora clearly reflects a unique phenomenon called selection which causes disappearance of all radiosensitive and survival of radioresistant types of microbes. There is indeed an increased number of radioresistant types of microbes with intensified activity of catalase and nuclease in pool water of atomic reactors.
